Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22072] to ASPA - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ASPA antibody [EPR22072] - BSA and Azide free
    See all ASPA primary antibodies

  • Description

    Rabbit monoclonal [EPR22072] to ASPA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum tissue.

  • General notes

    ab239522 is the carrier-free version of ab223269.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab223269).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of rat cerebrum. (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunoprecipitation - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunoprecipitation - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    ASPA was immunoprecipitated from 0.35 mg human brain lysate with ab223269 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223269 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human brain lysate 10 µg (Input).
    Lane 2: ab223269 IP in human brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223269 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of rat cerebral cortex (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining mainly on proximal renal tubules of human kidney (PMID: 16935940). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of human cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223269).

  • Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)
    Anti-ASPA antibody [EPR22072] - BSA and Azide free (ab239522)

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