Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ASPA antibody [EPR22072] (ab223269)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22072] to ASPA
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-ASPA antibody [EPR22072]
See all ASPA primary antibodies
Description

Rabbit monoclonal [EPR22072] to ASPA
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain, fetal kidney and testis lysate. Mouse brain lysate. Rat brain and testis lysate. IHC-P: Mouse and human cerebrum, rat cerebral cortex and human kidney tissues. IHC-Fr: Rat and mouse cerebrum tissues. IP: Human brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22072
Isotype

IgG
Research areas

Western blot - Anti-ASPA antibody [EPR22072] (ab223269)

All lanes : Anti-ASPA antibody [EPR22072] (ab223269) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human testis lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time : Lanes 1-3: 15 seconds; Lanes 4-6: 8 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of rat cerebrum. (PMID: 29116375).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-ASPA antibody [EPR22072] (ab223269)

ASPA was immunoprecipitated from 0.35 mg human brain lysate with ab223269 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223269 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Human brain lysate 10 µg (Input).
Lane 2: ab223269 IP in human brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223269 in human brain lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of rat cerebral cortex (PMID: 29116375). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining mainly on proximal renal tubules of human kidney (PMID: 16935940). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of human cerebrum (PMID: 29116375). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-ASPA antibody [EPR22072] (ab223269)

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