Anti-P4HA2 antibody (ab70887)
Key features and details
- Rabbit polyclonal to P4HA2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-P4HA2 antibody
See all P4HA2 primary antibodies -
Description
Rabbit polyclonal to P4HA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)
-
Positive control
- HeLa and 293T whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-P4HA2 antibody (ab70887)All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 28 kDa, 33 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-P4HA2 antibody (ab70887)HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-P4HA2 antibody (ab70887) Image courtesy of an anonymous Abreview.All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 35 kDa (possible degradation product)
Exposure time: 5 minutes
Good immunoreactivity observed in RIE-1 cell lysate that is consistent with the mobility of full-length P4HA2. A faster migrating species of ~35 kDa has been commonly observed with other antibodies (including this one) that could be a degradation product or alternatively spliced form of P4HA2. Further charcaterisation is required.
