Anti-P4HA2 antibody (ab233197)
Key features and details
- Rabbit polyclonal to P4HA2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P4HA2 antibody
See all P4HA2 primary antibodies -
Description
Rabbit polyclonal to P4HA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human P4HA2 aa 200 to the C-terminus. Two N-terminal tags. (Expressed in E.coli).
Database link: O15460 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Human pancreas tissue. WB: Recombinant human P4HA2 protein; HeLa and HEK-293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-P4HA2 antibody (ab233197)All lanes : Anti-P4HA2 antibody (ab233197) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HA2 antibody (ab233197)Formalin-fixed, paraffin-embedded human pancreas tissue stained for P4HA2 using ab233197 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-P4HA2 antibody (ab233197)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HA2 using ab233197 at 2 µg/ml in ICC/IF. FITC staining.
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Western blot - Anti-P4HA2 antibody (ab233197)Anti-P4HA2 antibody (ab233197) at 2 µg/ml + Recombinant human P4HA2 protein
