Anti-CBS antibody [EPR8578] (ab131155)
![Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-384474-anti-cbs-antibody-epr8578-western-blot-hela-raji.jpg "Anti-CBS antibody [EPR8578] (ab131155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8578] to CBS
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CBS antibody [EPR8578]
See all CBS primary antibodies -
Description
Rabbit monoclonal [EPR8578] to CBS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to residues near the N terminus of Human CBS (UniProt P35520).
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Positive control
- WB: Human fetal liver tissue lysate; HeLa and Raji cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.78 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8578 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CBS antibody [EPR8578] (ab131155)All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CBS knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-145421-anti-cbs-antibody-epr8578-western-blot.jpg)
Western blot - Anti-CBS antibody [EPR8578] (ab131155)All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
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![OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-145420-anti-cbs-antibody-epr8578.jpg)
OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155) -
![Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-4-benefits-of-recombinant-antibodies.png)
Anti-CBS antibody [EPR8578] (ab131155)
