Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBS antibody [EPR8579] (ab140600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8579] to CBS
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human, Pig

Overview

  • Product name

    Anti-CBS antibody [EPR8579]
    See all CBS primary antibodies

  • Description

    Rabbit monoclonal [EPR8579] to CBS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CBS aa 450-550. The exact sequence is proprietary.

  • Positive control

    • WB: Recombinant Human CBS protein (ab132564), human fetal liver tissue lysate, Raji, HeLa, and MCF7 cell lysates. IHC-P: Human pancreas tissue. ICC/IF: MCF7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CBS antibody [EPR8579] (ab140600)
    Western blot - Anti-CBS antibody [EPR8579] (ab140600)
    All lanes : Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CBS knockout HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab140600 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab140600 Anti-CBS antibody [EPR8579] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab140600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-CBS antibody [EPR8579] (ab140600)
    Western blot - Anti-CBS antibody [EPR8579] (ab140600)
    All lanes : Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

    Lane 1 : Human fetal liver cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR8579] (ab140600)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR8579] (ab140600)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling CBS with ab140600 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody [EPR8579] (ab140600)
    Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody [EPR8579] (ab140600)
    Immunofluorescent analysis of MCF7 cells labelling CBS with ab140600 at 1/100 dilution.
  • Anti-CBS antibody [EPR8579] (ab140600)
    Anti-CBS antibody [EPR8579] (ab140600)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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