Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10330] to LAP3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-LAP3 antibody [EPR10330] - BSA and Azide free
    See all LAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10330] to LAP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HepG2 and HeLa cell lysates.

  • General notes

    ab249116 is the carrier-free version of ab154809 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : 293T lysate
    Lane 3 : HepG2 lysate
    Lane 4 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using ab154809, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)

    This data was developed using ab154809, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)

    This data was developed using ab154809, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Flow Cytometry - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    This data was developed using ab154809, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    This data was developed using ab154809, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    This data was developed using ab154809, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    This data was developed using ab154809, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : LAP3 knockout A549 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab154809).

    Lanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
    Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)

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