Anti-CBS antibody (ab96252)
Key features and details
- Rabbit polyclonal to CBS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-CBS antibody
See all CBS primary antibodies -
Description
Rabbit polyclonal to CBS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig, Zebrafish
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 39-221 of Human CBS (AAH00440).
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Positive control
- WB: HeLa, Molt-4 and Raji whole cell lysates IHC-P: OVCAR3 xenograft; ICC: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : CBS Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 61 kDa
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All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : Molt-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.
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All lanes : Anti-CBS antibody (ab96252) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Human epithelial carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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HeLa cells stained for CBS (green) using ab96252 at 1/200 dilution in ICC/IF.