Anti-CBS antibody (ab135626)
Key features and details
- Rabbit polyclonal to CBS
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBS antibody
See all CBS primary antibodies -
Description
Rabbit polyclonal to CBS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 308-338 of Human CBS
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Positive control
- Mouse kidney tissue lysates, Human brain tissue and 293 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab135625 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-CBS antibody (ab135626) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg
Predicted band size: 61 kDa
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All lanes : Anti-CBS antibody (ab135626) at 1/100 dilution
Lane 1 : nontransfected 293 cells lysate
Lane 2 : lysate of 293 cells transiently transfected with the CBS gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 61 kDa
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Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labelling CBS with ab135626 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
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Flow cytometric analysis of ab135626 staining CBS at 1/10 in 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of CBS using ab135626 at 1/10 dilution in 293 cell followed by Alexa Fluor 488 conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).