Human MVP knockout HeLa cell lysate (ab257544)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260277 - Human MVP knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human MVP knockout HeLa cell lysate (ab257544)
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (20 ug)
Lane 2: MVP knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (20 ug)
Lane 3: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate (20 ug)
Lanes 1-3: Merged signal (red and green). Green - ab273093 observed at 110 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
ab273093 Anti-MVP antibody [EPR23594-106] was shown to specifically react with MVP in wild-type HeLa cells in Western blot. Significant decrease (8.7 % of intensity compared to the WT band) of signal was observed when MVP knockout cell line ab264817 (knockout cell lysate ab257544) was used.
ab273093 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human MVP knockout HeLa cell lysate (ab257544)Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: MVP knockout HeLa cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: MOLT-4 cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab177145 observed at 99 kDa. Red - loading control ab8245 observed at 37 kDa.
ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human MVP knockout HeLa cell lysate (ab257544)Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: MVP knockout HeLa cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: MOLT-4 cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab175239 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.
ab175239 Anti-MVP antibody [EPR13227(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab175239 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human MVP knockout HeLa cell lysate (ab257544)Allele-1: 17 bp deletion in exon 6
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Sanger Sequencing - Human MVP knockout HeLa cell lysate (ab257544)Allele-2: 1 bp deletion in exon 6
