Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10808(B)] to NUP133 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free
    See all NUP133 primary antibodies

  • Description

    Rabbit monoclonal [EPR10808(B)] to NUP133 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    ab236010 is the carrier-free version of ab155990 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236010 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010) This image is courtesy of an Abreview submitted by Aaron Halpen.

    Unpurified ab155990 staining NUP133 in mokey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)

    Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155990).

  • Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)
    Anti-NUP133 antibody [EPR10808(B)] - BSA and Azide free (ab236010)

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