Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1929Y] to Catalase - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Catalase antibody [EP1929Y] - BSA and Azide free
    See all Catalase primary antibodies

  • Description

    Rabbit monoclonal [EP1929Y] to Catalase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: human brain tissue. ICC/IF: HeLa cells.

  • General notes

    ab227116 is the carrier-free version of ab76024 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab227116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CAT knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab76024).

    Lanes 1-2: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab76024 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76024 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CAT knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 60 kDa



    This WB data was generated using the same anti-Catalase antibody clone, EP1929Y, in a different buffer formulation (cat# ab76024).

    Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

    Immunohistochemical analysis of Paraffin-embedded human bladder cancer tissue sections labeling Catalase with ab76024 at 1/1000. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Granular cytoplasmic staining on human bladder cancer.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Catalase ab76024 at 1/100. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

    ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/100 was used as counterstain antibody.

    Confocal image showing membranous staining in HeLa cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

    Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

  • Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
    Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

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