Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1C7] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free
  • Suitable for: IP, WB, ChIP, ELISA, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free
    See all RNA polymerase II CTD repeat YSPTSPS primary antibodies

  • Description

    Rat monoclonal [1C7] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IP, WB, ChIP, ELISA, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Other Immunogen Type. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Raji and RAW264.7, PC-12 lysates. IP: and Raji, RAW264.7 cells.

  • General notes

    ab255848 is the carrier-free version of ab252854. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] (ab252854) at 1/1000 dilution

    Lane 1 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 217 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID: 22745433)

    This blot was developed using a higher sensitivity ECL substrate. 

    Exposure time: 37 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

    Chromatin was prepared from HeLa cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min. 
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab252854 (red), or 5 µg of Rat IgG2a ab18450 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). 
    http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

    RNA polymerase II CTD repeat YSPTSPS was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab252854 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252854 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug

    Lane 2: ab252854 IP in RAW264.7 whole cell lysate

    Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252854 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

    ELISA analysis using ab252854 at a range of 4000-0 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) at 1/1000 dilution.

    Antigens: phospho S2, phospho S5, phospho T4, phospho Y1, phospho S7, non-phospho

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

    Dot blot analysis of ab252854 at 1/1000 dilution followed by a Goat Anti-Rat IgG (H+L), HRP) (ab205720) Secondary antibody at 1/5000 diluton. The observed data is consistent with what has been described in the literature (PMID: 22549466). Exposure trime: 3 minutes.

    Blocking and diluting buffer: 5% NFDM/TBST

    Lane 1: RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide 
    Lane 2: RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide 
    Lane 3: RNA polymerase II CTD repeat YSPTSPS (phospho T4) peptide 
    Lane 4: RNA polymerase II CTD repeat YSPTSPS (phospho Y1) peptide 
    Lane 5: RNA polymerase II CTD repeat YSPTSPS (phospho S7) peptide 
    Lane 6: RNA polymerase II CTD repeat YSPTSPS non-phopho peptide 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] (ab252854) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 217 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID: 22745433).

    Exposure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Immunoprecipitation - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

    RNA polymerase II CTD repeat YSPTSPS was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab252854 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252854 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 ug

    Lane 2: ab252854 IP in Raji whole cell lysate

    Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252854 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252854).

  • Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)
    Anti-RNA polymerase II CTD repeat YSPTSPS antibody [1C7] - BSA and Azide free (ab255848)

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