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Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free
    See all RAB12 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate.

  • General notes

    ab256765 is the carrier-free version of ab256487 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256765 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)
    Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)
    All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

    Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate at 20 µg
    Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 27 kDa


    Exposure time: 48 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256487).

    This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).

    MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

    The lysates were kindly provided by Professor Dario Alessi from University of Dundee.

    The antibody also reacts with unidentifiable proteins greater than 60 kDa.

    Blocking/Dilution buffer: AdvanBlock.

     

  • Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)
    Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)

    Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.

    Lane 1: RAB12 (phospho S106) peptide.

    Lane 2: RAB12 non-phospho peptide.

    Exposure time: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256487).

  • Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)
    Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)

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