Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Recombinant fragment
Overview
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Product name
Anti-RAB12 (phospho S106) antibody [MJF-R25-9]
See all RAB12 primary antibodies -
Description
Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R25-9 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate
Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 48 secondsThis antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).
MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The lysates were kindly provided by Professor Dario Alessi from University of Dundee.
The antibody also reacts with unidentifiable proteins greater than 60 kDa.
Blocking/Dilution buffer: AdvanBlock.
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Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.
Lane 1: RAB12 (phospho S106) peptide.
Lane 2: RAB12 non-phospho peptide.
Exposure time: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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