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Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5819(2)] to eIF3A - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free
    See all eIF3A primary antibodies

  • Description

    Rabbit monoclonal [EPR5819(2)] to eIF3A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248250 is the carrier-free version of ab128996. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248250 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    Western blot - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC-12 cell lysate
    Lane 7 : L6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 166 kDa



    This data was developed using ab128996, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    This data was developed using ab128996, the same antibody clone in a different buffer formulation.ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    Immunocytochemistry - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    This data was developed using ab128996, the same antibody clone in a different buffer formulation.ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.
  • OI-RD Scanning - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    OI-RD Scanning - Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    This data was developed using ab128996, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)
    Anti-eIF3A antibody [EPR5819(2)] - BSA and Azide free (ab248250)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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