Anti-PERK antibody [EPR19876-294] (ab229912)
![Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-344446-229912WB2.jpg "Anti-PERK antibody [EPR19876-294] (ab229912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19876-294] to PERK
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PERK antibody [EPR19876-294]
See all PERK primary antibodies -
Description
Rabbit monoclonal [EPR19876-294] to PERK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, HeLa, U-87 MG, HEK-293T, MCF7, RAW 264.7, NIH/3T3 and PC-12 whole cell lysates; Mouse brain lysate; Rat brain and liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19876-294 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 37 seconds; Lanes 3-6: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody also recognizes unidentifiable proteins below 37 kDa.
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![Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-344445-229912WB1.jpg)
Western blot - Anti-PERK antibody [EPR19876-294] (ab229912)All lanes : Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PERK knockout HAP1 whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 125 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes unidentifiable proteins below 75 kDa.
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![Anti-PERK antibody [EPR19876-294] (ab229912)](https://www.abcam.com/ps/products/229/ab229912/Images/ab229912-3-benefits-of-recombinant-antibodies.png)
Anti-PERK antibody [EPR19876-294] (ab229912)