Anti-Bile salt-activated lipase antibody (ab87431)
Key features and details
- Rabbit polyclonal to Bile salt-activated lipase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Bile salt-activated lipase antibody
See all Bile salt-activated lipase primary antibodies -
Description
Rabbit polyclonal to Bile salt-activated lipase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Drosophila melanogaster, Non human primates
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Immunogen
Synthetic peptide corresponding to Human Bile salt-activated lipase aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87432) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Pancreas; Mouse Pancreas
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab87431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa). IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Target
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Relevance
Defects in Bile salt activated lipase (CEL) are a cause of maturity onset diabetes of the young type 8 with exocrine dysfunction (MODY8) [MIM:609812], also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY [MIM:606391] is an autosomal dominant form of diabetes mellitus. The pancreas serves both endocrine and exocrine functions. The endocrine cells are found in the islets of Langerhans. They synthesize insulin and other hormones, and are involved in the pathogenesis of diabetes mellitus. The exocrine cells produce bicarbonate and digestive enzymes and are involved in the pathogenesis of pancreatic malabsorption. The localization of the islets within exocrine pancreatic tissue is suggestive of an interdependency and cross talk between these two cell populations in their normal and in their abnormal function. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 280748 Cow
- Entrez Gene: 1056 Human
- Entrez Gene: 12613 Mouse
- Entrez Gene: 24254 Rat
- Omim: 114840 Human
- SwissProt: P30122 Cow
- SwissProt: P19835 Human
- SwissProt: Q64285 Mouse
see all -
Alternative names
- BAL antibody
- Bile salt-stimulated lipase antibody
- BSDL antibody
see all
Images
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Western blot - Anti-Bile salt-activated lipase antibody (ab87431)All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa (possible cleavage fragment)
Exposure time: 30 seconds
We hypothesise that the 74 kDa band represents the short isoform of bile salt activated lipase, and the 72 kDa band represents this protein without its signal sequence. -
Western blot - Anti-Bile salt-activated lipase antibody (ab87431)All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 100,74 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa (possible cleavage fragment)
Exposure time: 30 seconds
The same batches of ab87431 detect bands at different molecular weights in different batches of human pancreas tissue lysate. When we tested ab87431 against a previous batch of human pancreas tissue lysate, we observed bands around 74-kDa. However, when the same antibody is tested against our current batch of this lysate, we see bands around 100-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bile salt-activated lipase antibody (ab87431)IHC image of Bile salt-activated lipase staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87431, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
References (0)
ab87431 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Bile salt-activated lipase antibody (ab87431)All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa (possible cleavage fragment)
Exposure time: 30 seconds
We hypothesise that the 74 kDa band represents the short isoform of bile salt activated lipase, and the 72 kDa band represents this protein without its signal sequence. -
Western blot - Anti-Bile salt-activated lipase antibody (ab87431)All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 100,74 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa (possible cleavage fragment)
Exposure time: 30 seconds
The same batches of ab87431 detect bands at different molecular weights in different batches of human pancreas tissue lysate. When we tested ab87431 against a previous batch of human pancreas tissue lysate, we observed bands around 74-kDa. However, when the same antibody is tested against our current batch of this lysate, we see bands around 100-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bile salt-activated lipase antibody (ab87431)IHC image of Bile salt-activated lipase staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87431, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
