Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neurturin antibody [EPR23301-10] (ab274417)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23301-10] to Neurturin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neurturin antibody [EPR23301-10]
    See all Neurturin primary antibodies

  • Description

    Rabbit monoclonal [EPR23301-10] to Neurturin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart, testis and brain tissue lysates; Mouse embryo and heart tissue lysates; Rat heart and brain tissue lysates; SH-SY5Y and Neuro-2a whole cell lysates. IHC-P: Human cerebrum and testis tissue; Mouse testis tissue; Rat testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23301-10

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 25841760). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

    Lane 1 : Mouse embryo tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa
    Observed band size: 16,28 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-3 were developed using a higher sensitivity ECL substrate.

    16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

    Exposure times: Lane 1-3: 3 minutes Lane 4-5: 37 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 3 : Human brain tissue lysate
    Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 16,28 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 10415380). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Neurturin antibody [EPR23301-10] (ab274417)
    Anti-Neurturin antibody [EPR23301-10] (ab274417)

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