Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14931] to PSME2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, ELISA, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PSME2 antibody [EPR14931] - BSA and Azide free
    See all PSME2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14931] to PSME2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Mouse
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, PC3. PC12, Neuro-2a, 4T1, and HepG2 whole cell lysates (ab7900); IHC-P: Human kidney clear cell carcinoma and stomach tissues; ICC: HeLa and HepG2 cells. Flow Cyt: HepG2 and Neuro-2a cells.

  • General notes

    Ab250700 is the carrier-free version of ab183727. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250700 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14931

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 3 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    This data was developed using ab183727, the same antibody clone in a different buffer formulation.

    Exposure times:

    Lane 1: 3.25 seconds

    Lane 2: 3 minutes

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)

    This data was developed using ab183727, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    This data was developed using ab183727, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC3 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab183727, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    This data was developed using ab183727, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Immunocytochemistry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    This data was developed using ab183727, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Immunocytochemistry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Immunocytochemistry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    This data was developed using ab183727, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)

    This data was developed using ab183727, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • ELISA - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    ELISA - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)

    This data was developed using ab183727, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human PSME2 recombinant protein at 250 ng/mL with ab183727. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Flow Cytometry - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    This data was developed using ab183727, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSME2 with ab183727 at 1/210 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)
    Anti-PSME2 antibody [EPR14931] - BSA and Azide free (ab250700)

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