Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13660] to Importin4/Imp4
Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-Importin4/Imp4 antibody [EPR13660]
See all Importin4/Imp4 primary antibodies
Description

Rabbit monoclonal [EPR13660] to Importin4/Imp4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human Importin4/Imp4. The exact sequence is proprietary.
Database link: Q8TEX9
Positive control
- WB: HepG2, HeLa, A549 and Human seminoma lysates. IHC-P: Human breast carcinoma tissue and Human testis tissue. ICC/IF: A549 cells. Flow Cyt: Permeabilized HepG2 cells.
General notes
This product was previously labelled as Importin4

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13660
Isotype

IgG
Research areas

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

All lanes : Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : IPO4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 119 kDa
Observed band size: 130 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab181046 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181046 Anti-Importin4/Imp4 antibody [EPR13660] was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266175 (knockout cell lysate ab258005) was used. Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE. ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Immunofluorescent staining of A549 cells labeling Importin4/Imp4 using ab181046 at a 1/50 dilution (red) and DAPI nuclear staining (blue).
Flow Cytometry - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Flow cytometric analysis of permeabilized HepG2 cells labeling Importin4/Imp4 using ab181046 at a 1/10 dilution (red) or a rabbit IgG control (green).
Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

All lanes : Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/100000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Human seminoma lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 119 kDa
Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labelling Importin4/Imp4 immunoprecipitated using ab181046 at a 1/10 dilution.
Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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