Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1353Y] to Importin 9/RANBP9
  • Suitable for: WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Importin 9/RANBP9 antibody [EP1353Y]
    See all Importin 9/RANBP9 primary antibodies

  • Description

    Rabbit monoclonal [EP1353Y] to Importin 9/RANBP9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Importin 9/RANBP9 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q96P70

  • Positive control

    • WB: HeLa and SK-BR-3 cell lysates; Fetal brain lysate. IHC-P: Human lymphoma tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Importin 9.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    All lanes : Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IPO9 knockout HeLa cell lysate
    Lane 3 : SK-BR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 115 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab52605. Red - loading control, ab8245 observed at 36 kDa. 

    ab52605 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab52605 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Flow Cytometry - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Overlay histogram showing HeLa cells stained with ab52605 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52605, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Western blot - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605) at 1/1000 dilution + Fetal brain lysate at 10 µg

    Secondary
    HRP-labelled goat anti-rabbit. at 1/2000 dilution

    Predicted band size: 115 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)

    Immunohistochemical staining of paraffin-embedded human lymphoma using ab52605 at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)
    Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab52605)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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