Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
![Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-392195-anti-ipo9-epr1352-antibody-western-blot-wt-hela-ipo9-ko-hela-sk-br-3-cells.jpg "Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Importin 9/RANBP9 antibody [EPR1352]
See all Importin 9/RANBP9 primary antibodies -
Description
Rabbit monoclonal [EPR1352] to Importin 9/RANBP9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human Importin 9/RANBP9 aa 50-150.
Database link: Q96P70 -
Positive control
- HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
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General notes
This product was previously labelled as Importin 9
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.29 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1352 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IPO9 knockout HeLa cell lysate
Lane 3 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 116 kDaLanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.
ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138511-anti-importin-9-antibody-epr1352-western-blot.jpg)
Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138510-anti-importin-9-antibody-epr1352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-138509-anti-importin-9-antibody-epr1352.jpg)
OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) -
![Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)](https://www.abcam.com/ps/products/124/ab124710/Images/ab124710-5-benefits-of-recombinant-antibodies.png)
Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
