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Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1352] to Importin 9/RANBP9
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Importin 9/RANBP9 antibody [EPR1352]
    See all Importin 9/RANBP9 primary antibodies

  • Description

    Rabbit monoclonal [EPR1352] to Importin 9/RANBP9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Importin 9/RANBP9 aa 50-150.
    Database link: Q96P70

  • Positive control

    • HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.

  • General notes

     

     

     This product was previously labelled as Importin 9

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IPO9 knockout HeLa cell lysate
    Lane 3 : SK-BR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 116 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa. 

    ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    All lanes : Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SKBR-3 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 116 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)
    Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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