Anti-ENT2 antibody [EPR11674] (ab181192)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11674] to ENT2
- Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-ENT2 antibody [EPR11674]
See all ENT2 primary antibodies -
Description
Rabbit monoclonal [EPR11674] to ENT2 -
Host species
Rabbit -
Specificity
May be unsuitable with mouse in WB. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, K562, Jurkat, SH-SY5Y cell lysates. Human RNT2 recombinant protein. Human clear cell carcinoma of the kidney tissue. HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11674 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma of the kidney tissue labeling ENT2 with ab181192 at 1/250 dilution and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution
Lane 1 : HepG2
Lane 2 : K562
Lane 3 : Jurkat
Lane 4 : SH-SY5Y
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 50 kDa
Additional bands at: 55-60 kDa (possible glycosylated form)
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Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ENT2 with ab181192 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green). Counterstained with DAPI (blue).
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Flow cytometric analysis of HepG2 cells fixed in 2% paraformaldehyde labeling ENT2 with ab181192 at 1/10 dilution and Goat anti-FITC at 1/150 dilution (pink). Rabbit monoclonal IgG was used as isotype control.
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Anti-ENT2 antibody [EPR11674] (ab181192) at 1/1000 dilution + Human ENT2 recombinant protein, 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
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