Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant S. cerevisiae KEX2 protease protein (ab204623)

Key features and details

  • Expression system: BTI-TN-5B1-4 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant S. cerevisiae KEX2 protease protein
    See all KEX2 protease proteins and peptides

  • Biological activity

    ab204623 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2. 1 milligram of ab204623 contains activity equivalent to at least 40 units of yeast derived KEX2. 

  • Purity

    > 95 % SDS-PAGE.
    ab204623 wasdetermined to be > 95% pure by SDS-PAGE & HPLC analyses.

  • Endotoxin level

  • Expression system

    BTI-TN-5B1-4 cells

  • Accession

    P13134

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Saccharomyces cerevisiae

    • Sequence

      LPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDL WYNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLS DDYHGTRCAGEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASL IYGLDV NDIYSCSWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAI YVFASGNGGT RGDNCNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAV MAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNL TWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEM SKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRI EHVTVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFM SVAHWGENGVGDWKIKVKTTEN GHRIDFHSWRLKLFGESIDSSKTETF VFGNDKEEVEPAATESTVSQYSASSTSISISATSTSSISIGVETSAIPQT TTASTDPDSDPNTP

    • Predicted molecular weight

      60 kDa

    • Amino acids

      110 to 667

    • Additional sequence information

      NP_014161.1. Lumenal domain.

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab204623 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        HPLC

        SDS-PAGE

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

        Lyophilized from 10 mM Sodium Acetate, pH 6.0 + 5 mM Calcium Chloride.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer (e.g. PBS) containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. The lyophilized protein is stable for 1 month at RT and for 6 months at 4°C. The reconstituted protein (diluted as CoA) is stable for 1 week at 4°C and for 12 months at -20°C to - 80°:

      General Info

      • Alternative names

        • KEX 2
        • Kex2p
        • Kexin KEX2
        • QDS1
        • SRB1
        • VMA45
        see all

      • Relevance

        Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

      • Cellular localization

        Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab204623? Please let us know so that we can cite the reference in this datasheet.

    ab204623 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • KEX 2
      • Kex2p
      • Kexin KEX2
      • QDS1
      • SRB1
      • VMA45
      see all

    • Relevance

      Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

    • Cellular localization

      Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

    Images

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