Mouse Interferon alpha 2 ELISA Kit (ab211648)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.9 pg/ml
- Range: 15.36 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Interferon alpha 2 ELISA Kit
See all Interferon alpha 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 2.2% Inter-assay Sample n Mean SD CV% Overall 3 2.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
0.9 pg/ml -
Range
15.36 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 94% - 100% Serum 94 93% - 97% Cell culture extracts 96 92% - 101% Hep Plasma 96 93% - 97% EDTA Plasma 96 95% - 98% Cit plasma 85 85% - 86% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Interferon alpha 2 ELISA Kit (ab211648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon alpha 2 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Interferon alpha 2 with 0.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
There are three major structural types of interferons: alpha, beta, and gamma. Interferon alpha forms are produced by monocytes/macrophages, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, and glucocorticoid hormones. Interferon alpha-2 is one of at least 23 different known variants of interferon alpha. The individual interferon alpha proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156 - 172 amino acids (aa). Mouse Interferon alpha-2 is 167 aa in length. Interferon alpha-2 demonstrates antiviral, antiparasitic, and antiproliferative activities.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Interferon alpha-2 Capture Antibody 1 x 600µl 10X Mouse Interferon alpha-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse Interferon alpha-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Alpha 2a interferon
- IFN
- IFN alpha 2
see all -
Database links
- Entrez Gene: 15965 Mouse
- SwissProt: P01573 Mouse
- Unigene: 14091 Mouse
Images
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Other - Mouse Interferon alpha 2 ELISA Kit (ab211648)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Interferon alpha-2 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Interferon alpha-2 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Interferon alpha-2 in mouse serum and plasma samples.The concentrations of Interferon alpha-2 were measured in duplicates, interpolated from the Interferon alpha-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 196.9 pg/mL in serum, 181.9 pg/mL in plasma (citrate), 204.1 pg/mL in plasma (EDTA), and 194.9 pg/mL in plasma (heparin).
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Interpolated concentrations of spiked Interferon alpha-2 in mouse cell culture supernatant samples.The concentrations of Interferon alpha-2 were measured in duplicates, interpolated from the Interferon alpha-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: 100% RAW 264.7 stimulated supernatant, 100% J774A.1 stimulated supernatant, and 100% PBMC stimulated supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 466.4 pg/mL in spiked RAW 264.7 stimulated supernatant, 500.1 pg/mL in spiked J774A.1 stimulated supernatant, and 518.9 pg/mL in spiked PBMC stimulated supernatant. RAW 264.7 supernatant was stimulated for 48 hours with 5 µg/mL of LPS. J774A.1 supernatant was stimulated for 72 hours with 1.5% PHA and 10 ng/mL PMA. PBMC supernatant was stimulated for 5 hours with 3 µg/mL of imiquimod.
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Interpolated concentrations of spiked Interferon alpha-2 in mouse J774A.1 and PBMC extracts based on a 50 µg/mL extract load.The concentrations of Interferon alpha-2 were measured in duplicate and interpolated from the Interferon alpha-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 435.6 pg/mL in spiked J774A.1 extract and 439.4 pg/mL in spiked PBMC extract.
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Interpolated concentrations of native Interferon alpha-2 in mouse J774A.1 extract based on a 50 µg/mL extract load.The concentrations of Interferon alpha-2 were measured in duplicate and interpolated from the Interferon alpha-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-2 concentration was determined to be 57.4 pg/mL in J774A.1 extract.
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Sandwich ELISA - Mouse Interferon alpha 2 ELISA Kit (ab211648)To learn more about the advantages of recombinant antibodies see here.
