C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (ab207199)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 500 ng/well
Properties
-
Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 1 x 11ml 10X Wash Buffer 1 x 22ml 1 x 110ml 96-well C/EBP assay plate 1 unit 5 units Anti-mouse HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 1 x 55µl Binding Buffer 1 x 10ml 1 x 50ml C/EBP alpha antibody 1 x 11µl 1 x 55µl c/EBP beta antibody 1 x 11µl 1 x 55µl Developing Solution 1 x 11ml 1 x 55ml Dithiothreitol (DTT) (1 M) 1 x 100µl 1 x 500µl Herring sperm DNA (1 μg/μL) 1 x 100µl 1 x 500µl Lysis Buffer 1 x 10ml 1 x 50ml Mutated oligonucleotide (20 pmol/µL) 1 x 100µl 1 x 500µl Plate sealer 1 unit 5 units Positive control nuclear extract (2.5 µg/µL) 1 x 40µl 1 x 200µl Protease Inhibitor Cocktail 1 x 100µl 1 x 500µl Stop Solution 1 x 11ml 1 x 55ml Wild-type oligonucleotide (20 pmol/µL) 1 x 100µl 1 x 500µl -
Research areas
-
Cellular localization
CEBP Alpha: Nucleus. CEBP Beta: Nucleus. -
Database links
- Entrez Gene: 1050 Human
- Entrez Gene: 1051 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 12608 Mouse
- Entrez Gene: 24252 Rat
- Entrez Gene: 24253 Rat
- Omim: 116897 Human
- Omim: 189965 Human
see all
Images
-
Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for C/EBP beta activation. These results are provided for demonstration purposes only.
Different amounts of unstimulated Jurkat (grey) and U-937 (black) were tested for C/EBP beta activation. These results are provided for demonstration purposes only.
-
Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for C/EBP alpha activation. These results are provided for demonstration purposes only.Different amounts of unstimulated Jurkat (grey) and U-937 (black) were tested for C/EBP alpha activation. These results are provided for demonstration purposes only.