Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant S. cerevisiae KEX2 protease protein (Active) (ab96554)

Key features and details

  • Expression system: BTI-TN-5B1-4 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: HPLC, SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant S. cerevisiae KEX2 protease protein (Active)
    See all KEX2 protease proteins and peptides

  • Biological activity

    ab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2.

    1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2.

    Cleaves at the carboxyl side of K/R-R.

  • Purity

    > 95 % SDS-PAGE.
    >= 95% HPLC analyses.

  • Endotoxin level

  • Expression system

    BTI-TN-5B1-4 cells

  • Accession

    P13134

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Saccharomyces cerevisiae

    • Sequence

      LPVPAPPMDS SLLPVKEAED KLSINDPLFE RQWHLVNPSF PGSDINVLDL WYNNITGAGV VAAIVDDGLD YENEDLKDNF CAEGSWDFND NTNLPKPRLS DDYHGTRCAG EIAAKKGNNF CGVGVGYNAK ISGIRILSGD ITTEDEAASL IYGLDVNDIY SCSWGPADDG RHLQGPSDLV KKALVKGVTE GRDSKGAIYV FASGNGGTRG DNCNYDGYTN SIYSITIGAI DHKDLHPPYS EGCSAVMAVT YSSGSGEYIH SSDINGRCSN SHGGTSAAAP LAAGVYTLLL EANPNLTWRD VQYLSILSAV GLEKNADGDW RDSAMGKKYS HRYGFGKIDA HKLIEMSKTW ENVNAQTWFY LPTLYVSQST NSTEETLESV ITISEKSLQD ANFKRIEHVT VTVDIDTEIR GTTTVDLISP AGIISNLGVV RPRDVSSEGF KDWTFMSVAH WGENGVGDWK IKVKTTENGH RIDFHSWRLK LFGESIDSSK TETFVFGNDK EEVEPAATES TVSQYSASST SISISATSTS SISIGVETSA IPQTTTASTD PDSDPNTP

    • Predicted molecular weight

      60 kDa

    • Amino acids

      110 to 667

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab96554 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        HPLC

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituents: 0.0555% Calcium chloride, 0.082% Sodium acetate

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.

      General Info

      • Alternative names

        • KEX 2
        • Kex2p
        • Kexin KEX2
        • QDS1
        • SRB1
        • VMA45
        see all

      • Relevance

        Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

      • Cellular localization

        Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab96554? Please let us know so that we can cite the reference in this datasheet.

    ab96554 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • KEX 2
      • Kex2p
      • Kexin KEX2
      • QDS1
      • SRB1
      • VMA45
      see all

    • Relevance

      Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.

    • Cellular localization

      Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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