Anti-HCN1 antibody [N70/28] (ab84816) + rat brain lysate

Predicted band size: 99 kDa
Observed band size: 99 kDa



As secondary antibody a goat anti-mouse IgG conjugated with HRP was used.

4% formalin-fixed human cerebellum tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

10% formalin-fixed frozen section of human brain tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

10% formalin-fixed frozen section of mouse brain tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

ab84816 staining HCN1 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).