Anti-HCN1 antibody - C-terminal (ab229340)
Key features and details
- Rabbit polyclonal to HCN1 - C-terminal
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HCN1 antibody - C-terminal
See all HCN1 primary antibodies -
Description
Rabbit polyclonal to HCN1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-Fr MouseIHC-P MouseRatWB MouseRatHuman
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Immunogen
Recombinant fragment within Human HCN1 (C terminal). The exact sequence is proprietary.
Database link: O60741 -
Positive control
- WB: Mouse and rat brain tissue extracts; SK-N-AS whole cell extract. IHC-P: Mouse retina and brain tissues; rat brain tissue. ICC/IF: Rat DIV9 E18 primary cortical neurons. IHC-Fr: Frozen mouse cerebellum and hippocampus tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HCN1 antibody - C-terminal (ab229340)Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution + SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-HCN1 antibody - C-terminal (ab229340)4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for HCN1 (green) using ab229340 at 1/500 dilution in ICC/IF.
Beta Tubulin 3/ Tuj1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (ab229340)Paraffin-embedded rat brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (ab229340)Paraffin-embedded mouse brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (ab229340)Frozen-sectioned adult mouse hippocampus tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue)
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Western blot - Anti-HCN1 antibody - C-terminal (ab229340)All lanes : Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (ab229340)Frozen-sectioned adult mouse cerebellum tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
beta Tubulin 3/ TUJ1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (ab229340)Paraffin-embedded mouse retina tissue stained for HCN1 using ab229340 at 1/800 dilution in immunohistochemical abalysis.
