Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE, WB, Functional Studies
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Product name
Recombinant human DNA Polymerase Kappa/POLK protein -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFY GNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRA AMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISP LLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFR IEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISL GLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKER LKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADF PHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKT DKDKFVKPLE -
Predicted molecular weight
64 kDa -
Amino acids
1 to 560 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C.
pH: 6
Constituents: 1.17% Sodium chloride, 0.14% Sodium phosphate, 50% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min. -
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)SDS-PAGE analysis of ab196956.