Anti-DNA polymerase alpha/POLA antibody (ab176734)
Key features and details
- Rabbit polyclonal to DNA polymerase alpha/POLA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies -
Description
Rabbit polyclonal to DNA polymerase alpha/POLA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human DNA polymerase alpha/POLA aa 1175-1225. The exact sequence is proprietary. NP_058633.2.
Database link: P09884 -
Positive control
- HeLa and 293T cell lysates
-
General notes
This product was previously labelled as DNA polymerase alpha
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176734 is affinity purified using an epitope specific to DNA polymerase alpha/POLA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DNA polymerase alpha/POLA antibody (ab176734)All lanes : Anti-DNA polymerase alpha/POLA antibody (ab176734) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-DNA polymerase alpha/POLA antibody (ab176734)ab176734 at 0.4 µg/ml detecting DNA polymerase alpha/POLA in HeLa whole cell lysate by WB following IP. ab176734 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
