Anti-DNA polymerase alpha/POLA antibody (ab31777)
Key features and details
- Rabbit polyclonal to DNA polymerase alpha/POLA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNA polymerase alpha/POLA antibody
See all DNA polymerase alpha/POLA primary antibodies -
Description
Rabbit polyclonal to DNA polymerase alpha/POLA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human DNA polymerase alpha/POLA aa 1100-1200.
(Peptide available asab30406) -
General notes
This product was previously labelled as DNA polymerase alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). Target
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Function
Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. -
Sequence similarities
Belongs to the DNA polymerase type-B family. -
Post-translational
modificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 418593 Chicken
- Entrez Gene: 480869 Dog
- Entrez Gene: 5422 Human
- Entrez Gene: 18968 Mouse
- Entrez Gene: 85241 Rat
- Entrez Gene: 398200 Xenopus laevis
- Omim: 312040 Human
- SwissProt: P09884 Human
see all -
Alternative names
- DKFZp686K1672 antibody
- DNA polymerase alpha 1 catalytic subunit antibody
- DNA polymerase alpha catalytic subunit antibody
see all
Images
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Western blot - Anti-DNA polymerase alpha/POLA antibody (ab31777)All lanes : Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Protocols
References (7)
ab31777 has been referenced in 7 publications.
- Abdel-Samad R et al. Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase a. Am J Cancer Res 8:39-55 (2018). PubMed: 29416919
- Bellelli R et al. Pole Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell 70:707-721.e7 (2018). PubMed: 29754823
- Kolinjivadi AM et al. Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments. Mol Cell 67:867-881.e7 (2017). PubMed: 28757209
- Aze A et al. RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin. Nat Commun 8:2130 (2017). PubMed: 29242643
- Yun J et al. Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. Nucleic Acids Res 44:558-72 (2016). PubMed: 26420833
- Hashimoto Y et al. Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis. Nat Struct Mol Biol 17:1305-11 (2010). PubMed: 20935632
- Ferguson RL & Maller JL Centrosomal localization of cyclin E-Cdk2 is required for initiation of DNA synthesis. Curr Biol 20:856-60 (2010). WB ; Chinese hamster . PubMed: 20399658
Images
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Western blot - Anti-DNA polymerase alpha/POLA antibody (ab31777)All lanes : Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
