Inhibition of BAFF-mediated splenocyte survival. ab157042 inhibits BAFF-mediated activation of freshly isolated splenocytes. Concentration of ab157042 to achieve complete inhibition depends on the concentration of soluble BAFF used. A ratio (soluble BAFF:ab157042) 1:50 and higher is recommended. Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS, and B cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone, with the indicated concentration of recombinant Human soluble BAFF alone or in the presence of an excess of ab157042 (50µg/ml). After three days in culture, cells were again analyzed on FACS with the saved setting. Splenocytes survival index (=ratio of %living/%dead cells) was calculated and plotted.