Human sTNF RI ELISA Kit (ab209881)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.9 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human sTNF RI ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 5 2.3% Inter-assay Sample n Mean SD CV% Human serum 3 6.1% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.9 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 107 102% - 116% Serum 101 87% - 110% Cell culture media 108 108% - 109% Hep Plasma 109 105% - 112% EDTA Plasma 99 89% - 105% Cit plasma 98 90% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human sTNF RI ELISA Kit (ab209881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RI protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human sTNF RI with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tumor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human sTNF R1 Capture Antibody 1 x 600µl 10X Human sTNF R1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human sTNF R1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Alternative names
- CD120a
- Soluble TNF p55 Receptor
- TNFRSF1A
see all -
Database links
- Entrez Gene: 7132 Human
- Omim: 191190 Human
- SwissProt: P19438 Human
- Unigene: 279594 Human
Images
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Other - Human sTNF RI ELISA Kit (ab209881)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human sTNF R1 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native sTNF R1 in human plasma samples.The concentrations of sTNF R1 were measured in duplicates, interpolated from the TNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 6.25%, plasma (EDTA) 6.25%, and plasma (heparin) 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 1,791 pg/mL in plasma (citrate), 2,282 pg/mL in plasma (EDTA) and 2,455 pg/mL in plasma (heparin).
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Interpolated concentrations of native sTNF R1 in human serum and urine samples.The concentrations of sTNF R1 were measured in duplicates, interpolated from the sTNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25% and urine 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,318 pg/mL in serum and 4,082 pg/mL in urine.
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Peripheral Blood Mononuclear Cells (PBMC) were grown in the absence (unstimulated) or presence of phytohemagglutinin M (PHA-M) for 2 days.Human sTNF R1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PHA-M stimulated PBMC and cell culture media. Measured values were interpolated from the sTNF R1 standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of the duplicate values +/- SD are graphed: 86.1 pg/mL unstimulated, 196.4 pg/mL stimulated and undetectable in media.
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Serum from ten individual healthy female human donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,043 pg/mL with a range of 1,500 – 2,509 pg/mL
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Sandwich ELISA - Human sTNF RI ELISA Kit (ab209881)To learn more about the advantages of recombinant antibodies see here.
