Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdc37 antibody [EPR4934] (ab109419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4934] to Cdc37
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdc37 antibody [EPR4934]
    See all Cdc37 primary antibodies

  • Description

    Rabbit monoclonal [EPR4934] to Cdc37

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, A431, K-562, LNCaP, NIH/3T3, Raw 264.7, PC-12, and C6 whole cell lysates. Rat and mouse heart tissue lysates; IP: Jurkat whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)
    Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)
    All lanes : Anti-Cdc37 antibody [EPR4934] (ab109419) at 1/10000 dilution (purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 9 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)
    Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)
    Anti-Cdc37 antibody [EPR4934] (ab109419) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (ab109419)
    Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (ab109419)

    Purified ab109419 at 1/20 dilution (0.5 µg) immunoprecipitating Cdc37 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg
    Lane 2 (+): ab109419 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109419 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 44 kDa

  • Anti-Cdc37 antibody [EPR4934] (ab109419)
    Anti-Cdc37 antibody [EPR4934] (ab109419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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