HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)
![HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)](https://www.abcam.com/ps/products/202/ab202364/Images/ab202364-258220-anti-cdc37-phospho-s13-antibody-epr4879-hrp-western-blot.jpg "HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cdc37 (phospho S13) antibody [EPR4879]
See all Cdc37 primary antibodies -
Description
HRP Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13) -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate and HeLa whole cell lysate serum starved and treated with Calyculin A.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4879 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)All lanes : HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) whole cell lysate serum starved and Calyculin A treated
Lane 3 : HeLa (Human epithelial carcinoma cell line) whole cell lysate, serum starved and Calyculin A treated. The membrane was then incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202864 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)](https://www.abcam.com/ps/products/202/ab202364/Images/ab202364-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab202364)
