Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RACK1 antibody [EPR7388] (ab129084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7388] to RACK1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RACK1 antibody [EPR7388]
    See all RACK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7388] to RACK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human RACK1 (UniProt ID: P63244).

  • Positive control

    • WB: HeLa, HepG2, SH-SY5Y, MCF7, C6, PC-12, Mouse brain, and NIH/3T3 lysates. IHC-P: human colon carcinoma, and cervical carcinoma, mouse cerebrum, and rat cerebrum tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
    Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
    All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 6 : Mouse brain lysates
    Lane 7 : Mouse spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
    Western blot - Anti-RACK1 antibody [EPR7388] (ab129084)
    All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACK1 antibody [EPR7388] (ab129084)

    ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RACK1 antibody [EPR7388] (ab129084)
    Anti-RACK1 antibody [EPR7388] (ab129084)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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