Anti-Ceramide synthase 1/LAG1 antibody (ab85696)
Key features and details
- Goat polyclonal to Ceramide synthase 1/LAG1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ceramide synthase 1/LAG1 antibody
See all Ceramide synthase 1/LAG1 primary antibodies -
Description
Goat polyclonal to Ceramide synthase 1/LAG1 -
Host species
Goat -
Specificity
ab85696 is expected to recognize both reported isoforms (NP_067090.1 and NP_937850.1). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Ceramide synthase 1/LAG1 aa 318-329 (internal sequence). (NP_937850.1)
Sequence:C-KDLREYDTAEAQ
Database link: NP_067090.1 -
Positive control
- Human brain (cerebellum, cerebral cortex and frontal cortex) lysates.
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General notes
This product was previously labelled as Ceramide synthase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85696 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ceramide synthase 1/LAG1 antibody (ab85696)Anti-Ceramide synthase 1/LAG1 antibody (ab85696) at 1 µg/ml + Human Frontal cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. An additional band of unknown identity was consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide.
