Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATG3 antibody [EPR4802] - BSA and Azide free (ab247616)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4802] to ATG3 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATG3 antibody [EPR4802] - BSA and Azide free
    See all ATG3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4802] to ATG3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa and K562 cell lysates.

  • General notes

    Ab247616 is the carrier-free version of ab108282. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247616 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-ATG3 antibody [EPR4802] - BSA and Azide free (ab247616)
    Western blot - Anti-ATG3 antibody [EPR4802] - BSA and Azide free (ab247616)
    All lanes : Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ATG3 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab108282).

    Lanes 1-4: Merged signal (red and green). Green - ab108282 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa. 

     ab108282 Anti-ATG3 antibody [EPR4802] was shown to specifically react with ATG3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266707 (knockout cell lysate ab257363) was used.  Wild-type and ATG3 knockout samples were subjected to SDS-PAGE.  ab108282 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-ATG3 antibody [EPR4802] - BSA and Azide free (ab247616)
    Anti-ATG3 antibody [EPR4802] - BSA and Azide free (ab247616)

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