Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Cdc37 (phospho S13) antibody [EPR4879]
    See all Cdc37 primary antibodies

  • Description

    Rabbit monoclonal [EPR4879] to Cdc37 (phospho S13)

  • Host species

    Rabbit

  • Specificity

    ab108360 only detects Cdc37 phosphorylated at Serine 13.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HeLa (treated with fetal bovine serum and Calyculin A) and HEK293 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/5000 dilution

    Lane 1 : Untreated HeLa whole cell lysates
    Lane 2 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates
    Lane 3 : HeLa were starved for 24 hours, then cultured in medium with 20% fetal bovine serum (FBS) for 5 hours, and then treated with 100ng/ml Calyculin A for 30 min whole cell lysates, the membrane was then incubated with Alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking buffer and concentration 2% BSA/TBST
    Diluting buffer and concentration 2% BSA/TBST

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

    Lane 1 : HeLa whole cell lysate - treated with fetal bovine serum and Calyculin A
    Lane 2 : HeLa whole cell lysate - untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360) at 1/20000 dilution

    Lane 1 : HEK293 whole cell lysate - untreated
    Lane 2 : HEK293 whole cell lysate - treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Dot Blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

    Dot blot analysis of Cdc37 (pS13) phospho peptide (lane 1) and Cdc37 non-phospho peptide (lane 2) labelling Cdc37 (phospho S13) with ab108360 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Western blot - Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    All lanes : Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

    Lane 1 : Jurkat cell lysate (untreated)
    Lane 2 : Jurkat cell lysate (treated with Alkaline Phosphatase)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)
    Anti-Cdc37 (phospho S13) antibody [EPR4879] (ab108360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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