Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

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Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23057-116] to LATS1/WARTS
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-LATS1/WARTS antibody [EPR23057-116]
See all LATS1/WARTS primary antibodies
Description

Rabbit monoclonal [EPR23057-116] to LATS1/WARTS
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human LATS1 recombinant protein; NIH/3T3, HeLa and PC-12 whole cell lysates. Flow Cyt: HeLa and NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23057-116
Isotype

IgG
Research areas

Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

All lanes : Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 126 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1:81 seconds;

Lanes 2-3: 3 minutes

The molecular weight observed is consistent with what has been described in the literature (PMID: 30293781).

Bands below 150KD are likely to be degraded LATS1 (PMID: 23573293).

Lysates should be made fresh and used in WB immediately to minimize protein degradation.
Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Flow cytometric analysis of 4% paraformaldehyde, fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

All lanes : Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656) at 1/1000 dilution

Lane 1 : His-tagged human LATS1 recombinant protein (aa701-1054) 10 ng
Lane 2 : His-tagged human LATS2 recombinant protein (aa664-1016) 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 126 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Samples loaded are extracts of E.coil expressing either LATS1 or LATS2.
Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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