Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23057-116] to LATS1/WARTS
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LATS1/WARTS antibody [EPR23057-116]
    See all LATS1/WARTS primary antibodies

  • Description

    Rabbit monoclonal [EPR23057-116] to LATS1/WARTS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human LATS1 recombinant protein; NIH/3T3, HeLa and PC-12 whole cell lysates. Flow Cyt: HeLa and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    All lanes : Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 126 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1:81 seconds;

    Lanes 2-3: 3 minutes

    The molecular weight observed is consistent with what has been described in the literature (PMID: 30293781).

    Bands below 150KD are likely to be degraded LATS1 (PMID: 23573293).

    Lysates should be made fresh and used in WB immediately to minimize protein degradation.

     

  • Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

    Flow cytometric analysis of 4% paraformaldehyde, fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    All lanes : Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656) at 1/1000 dilution

    Lane 1 : His-tagged human LATS1 recombinant protein (aa701-1054) 10 ng
    Lane 2 : His-tagged human LATS2 recombinant protein (aa664-1016) 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 126 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Samples loaded are extracts of E.coil expressing either LATS1 or LATS2.

  • Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    Flow Cytometry - Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling LATS1/WARTS with ab243656 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)
    Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-LATS1/WARTS antibody [EPR23057-116] (ab243656)

© 2021 Astana Biomed Group LLP. All rights reserved.