Anti-LATS1/WARTS antibody (ab70562)
Key features and details
- Rabbit polyclonal to LATS1/WARTS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies -
Description
Rabbit polyclonal to LATS1/WARTS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
Database link: O95835 -
Positive control
- HeLa whole cell lysate
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General notes
This product was previously labelled as LATS1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70562 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LATS1/WARTS antibody (ab70562)All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 350 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
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Immunoprecipitation - Anti-LATS1/WARTS antibody (ab70562)Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1/WARTS was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1/WARTS antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70562 at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
