COS-7 nuclear extract lysate (CoCl2 treated) (ab14644)
Overview
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Product name
COS-7 nuclear extract lysate (CoCl2 treated)
See all COS7 lysates -
Description
COS-7 nuclear extract lysate -
General notes
The COS7 nuclear extracts have been collected in Lysis Buffer after a 16 hour incubation with or without CoCl2 (0.15 mM). The protein content has been determined by a Bradford based assay (2.50 mg/ml). We recommend aliquoting the extracts into single use fractions and then storing.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: Protease inhibitor, 0.00021% Sodium molybdate, 0.216% Beta glycerophosphate, 0.0122% Sodium metavanadate, 0.042% Sodium fluoride, 5% Beta mercaptoethanol, 1% Sodium lauryl sulfate, 0.0154% DTT, 0.476% HEPES, 2.92% EDTA, 20% Glycerol, 2.32% Sodium chloride -
Concentration information loading... -
Background
These fibroblast-like cells grow in monolayers and are possible hosts for the propagation of pure populations of recombinant SV40 virus and contain T antigen of SV 40 virus. They are useful in virus replication studies and transfection studies.