Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CRM1 antibody [EPR14123(B)] (ab180144)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14123(B)] to CRM1
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-CRM1 antibody [EPR14123(B)]
See all CRM1 primary antibodies
Description

Rabbit monoclonal [EPR14123(B)] to CRM1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CRM1 aa 50-150. The exact sequence is proprietary.
Database link: O14980
Positive control
- K562, HeLa, and Jurkat cell lysate. K562 and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14123(B)
Isotype

IgG
Research areas

Western blot - Anti-CRM1 antibody [EPR14123(B)] (ab180144)

All lanes : Anti-CRM1 antibody [EPR14123(B)] (ab180144) at 1/2000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 123 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5%NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-CRM1 antibody [EPR14123(B)] (ab180144)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling CRM1 with Purified ab180144 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Flow Cytometry - Anti-CRM1 antibody [EPR14123(B)] (ab180144)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CRM1 with ab180144 at 1/200. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.
Immunocytochemistry/ Immunofluorescence - Anti-CRM1 antibody [EPR14123(B)] (ab180144)

Immunofluorescent analysis of 20°C acetone fixed K562 cells labeling CRM1 with ab180144 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) .
Anti-CRM1 antibody [EPR14123(B)] (ab180144)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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