Anti-P Glycoprotein antibody - N-terminal (ab202976)
Key features and details
- Rabbit polyclonal to P Glycoprotein - N-terminal
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-P Glycoprotein antibody - N-terminal
See all P Glycoprotein primary antibodies -
Description
Rabbit polyclonal to P Glycoprotein - N-terminal -
Host species
Rabbit -
Specificity
The immunogen for ab202976 is derived from Mouse ABCB1A and is 75% cross-reactive with Mouse ABCB1B.
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Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Mouse P Glycoprotein aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. The immunogen for ab202976 is derived from Mouse ABCB1A.
Database link: P21447 -
Positive control
- WB: Mouse Liver lysates. SH-SY5Y, HEK-293T, and HepG2 cell lysates; ICC: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine)
Aqueous buffered solution. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-P Glycoprotein antibody - N-terminal (ab202976)
Immunocytochemistry analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling P Glycoprotein with ab202976 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with Triton X-100 at room temperature for 20 minutes. Normal goat serum was used as the blockng buffer at 37°C for 20 minutes. Primary incuabtion was for 90 minutes at 37°C followed by a FITC conjugated Goat Anti-Rabbit IgG secondary antibody at 37°C for 90 minutes (green). Nuclear DNA was labelled with DAPI (blue).
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Western blot - Anti-P Glycoprotein antibody - N-terminal (ab202976)Anti-P Glycoprotein antibody - N-terminal (ab202976) at 1/300 dilution + Rat liver tissue lysate
Secondary
Conjugated secondary antibody at 1/10000 dilutionPrimary incubated at 4°C overnight followed by secondary antibody incubation for 60 minutes at 37°C.
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Western blot - Anti-P Glycoprotein antibody - N-terminal (ab202976)All lanes : Anti-P Glycoprotein antibody - N-terminal (ab202976) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : Conjugated secondary antibody at 1/20000 dilutionPrimary antibody incubation at 4°C overnight followed by secondary antibody incubation for 60 minutes at 37°C.
