Anti-TNPO3 antibody (ab71388)
Key features and details
- Rabbit polyclonal to TNPO3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TNPO3 antibody
See all TNPO3 primary antibodies -
Description
Rabbit polyclonal to TNPO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Xenopus laevis, Bird, Non human primates
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Immunogen
Synthetic peptide corresponding to Human TNPO3 aa 100-200.
(Peptide available asab71387) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated for 24hr, 500nM), Y79, Ramos, MEF1
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab71388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 104 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins SFRS1 and SFRS2, by recognizing phosphorylated RS domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23534 Human
- Entrez Gene: 320938 Mouse
- Omim: 610032 Human
- SwissProt: Q9Y5L0 Human
- SwissProt: Q6P2B1 Mouse
- Unigene: 193613 Human
- Unigene: 276381 Mouse
- Unigene: 304472 Mouse
see all -
Alternative names
- TNPO 3 antibody
- Imp12 antibody
- Importin 12 antibody
see all
Images
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Western blot - Anti-TNPO3 antibody (ab71388)All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-TNPO3 antibody (ab71388)ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.
Protocols
Datasheets and documents
References (2)
ab71388 has been referenced in 2 publications.
- De Iaco A et al. TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm. Retrovirology 10:20 (2013). WB . PubMed: 23414560
- De Iaco A & Luban J Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. Retrovirology 8:98 (2011). WB . PubMed: 22145813
Images
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Western blot - Anti-TNPO3 antibody (ab71388)All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
-
Immunocytochemistry/ Immunofluorescence - Anti-TNPO3 antibody (ab71388)ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.
