Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10808(B)] to NUP133
Suitable for: WB, ICC/IF
Reacts with: Human, African green monkey

Product name

Anti-NUP133 antibody [EPR10808(B)]
See all NUP133 primary antibodies
Description

Rabbit monoclonal [EPR10808(B)] to NUP133
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

African green monkey

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human NUP133.
Positive control
- HeLa, Ramos and SH-SY5Y cell lysates; HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human African green monkey

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10808(B)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 129 kDa
Observed band size: 129 kDa
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Lanes 1 & 3 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purified)
Lane 2 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purifed)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 129 kDa
Observed band size: 129 kDa
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)

All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 129 kDa

This image was produced using unpurified antibody.
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990) This image is courtesy of an Abreview submitted by Aaron Halpen

Unpurified ab155990 staining NUP133 in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor^® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.
Anti-NUP133 antibody [EPR10808(B)] (ab155990)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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