Anti-PAK1 antibody (ab183894)
Key features and details
- Rabbit polyclonal to PAK1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human PAK1 aa 151-544. The exact sequence is proprietary.
Database link: Q13153 -
Positive control
- Hepatoma tissue. A431 cells. HeLa, A431, BCL-1 and rat PC12 whole cell lysates/extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 antibody (ab183894)All lanes : Anti-PAK1 antibody (ab183894) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
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Western blot - Anti-PAK1 antibody (ab183894)Anti-PAK1 antibody (ab183894) at 1/1000 dilution + BCL-1 whole cell lysate/extract at 30 µg
Predicted band size: 61 kDa
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Western blot - Anti-PAK1 antibody (ab183894)Anti-PAK1 antibody (ab183894) at 1/1000 dilution + rat PC12 whole cell lysate/extract at 30 µg
Predicted band size: 61 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody (ab183894)Immunohistochemical analysis of paraffin-embedded human hepatoma labeling PAK1 using ab183894 at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-PAK1 antibody (ab183894)Immunofluorescence analysis of A431 cells fixed in ice-cold methanol for 5 minutes, labeling PAK1 using ab183894 at a 1/500 dilution (green). Blue: Hoechst 33343 staining.
