Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

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Anti-DISC1 antibody [EPR14684] (ab192258)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14684] to DISC1
Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-DISC1 antibody [EPR14684]
See all DISC1 primary antibodies
Description

Rabbit monoclonal [EPR14684] to DISC1
Host species

Rabbit
Specificity

This antibody has cross-reactivity with GST, so we suggest not to use this antibody for detecting protein with a GST-tag.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Molt-4, SH-SY5Y, U87-MG, C6, RAW264.7, PC12, NIH/3T3 and HeLa whole cell lysate (ab150035). Human brain, mouse brain and human adenocarcinoma of endometrium tissue. Neuro-2a cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14684
Isotype

IgG
Research areas

Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)

All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/10000 dilution

Lane 1 : MOLT-4 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

Immunohistochemical analysis of paraffin embedded mouse brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DISC1 antibody [EPR14684] (ab192258)

Immunofluorescent analysis of 4% formaldehyde fixed Neuro-2a cells labeling DISC1 using ab192258 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. Counterstained with DAPI. The two negative controls: 1. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.
Flow Cytometry - Anti-DISC1 antibody [EPR14684] (ab192258)

Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling DISC1 (red) with purified ab192258 at a 1/1000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-DISC1 antibody [EPR14684] (ab192258)

Lysate from U87-MG cells was immunoprecipitated with ab192258 at a 1/60 dilution. For the subsequent blot, ab192258 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)

All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

Immunohistochemical analysis of paraffin embedded human brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-DISC1 antibody [EPR14684] (ab192258)

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