Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DISC1 antibody [EPR14684] (ab192258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14684] to DISC1
  • Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DISC1 antibody [EPR14684]
    See all DISC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14684] to DISC1

  • Host species

    Rabbit

  • Specificity

    This antibody has cross-reactivity with GST, so we suggest not to use this antibody for detecting protein with a GST-tag.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Molt-4, SH-SY5Y, U87-MG, C6, RAW264.7, PC12, NIH/3T3 and HeLa whole cell lysate (ab150035). Human brain, mouse brain and human adenocarcinoma of endometrium tissue. Neuro-2a cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)
    Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)
    All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/10000 dilution

    Lane 1 : MOLT-4 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

    Immunohistochemical analysis of paraffin embedded mouse brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DISC1 antibody [EPR14684] (ab192258)
    Immunocytochemistry/ Immunofluorescence - Anti-DISC1 antibody [EPR14684] (ab192258)

    Immunofluorescent analysis of 4% formaldehyde fixed Neuro-2a cells labeling DISC1 using ab192258 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. Counterstained with DAPI.  The two negative controls: 1. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.

  • Flow Cytometry - Anti-DISC1 antibody [EPR14684] (ab192258)
    Flow Cytometry - Anti-DISC1 antibody [EPR14684] (ab192258)

    Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling DISC1 (red) with purified ab192258 at a 1/1000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-DISC1 antibody [EPR14684] (ab192258)
    Immunoprecipitation - Anti-DISC1 antibody [EPR14684] (ab192258)

    Lysate from U87-MG cells was immunoprecipitated with ab192258 at a 1/60 dilution. For the subsequent blot, ab192258 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)
    Western blot - Anti-DISC1 antibody [EPR14684] (ab192258)
    All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

    Immunohistochemical analysis of paraffin embedded human brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DISC1 antibody [EPR14684] (ab192258)

    Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-DISC1 antibody [EPR14684] (ab192258)
    Anti-DISC1 antibody [EPR14684] (ab192258)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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